Table 6.
Annotation results of new ATDs flanking sequences in heat-shocked plants from different HSP::TRANSPOSASE/ATDs double transgenic aspen lines without any phenotypic alterations ("blind" approach) grown in the greenhouse.
| Transgenic line | Number of plants | Plant affiliation | Genomic insertion locus (scaffold:position)* | Score | E-value | Transcript (POPTR_) | Functional annotation |
|---|---|---|---|---|---|---|---|
| # 82 | 16:6,171,964 | 41.0 | 5.7e-2 | ||||
| # 147 | 8:7,052,973 | 248.3 | 1.1e-64 | ||||
| N82-5 | 5 | # 210 | 9:11,936,320 | 114.9 | 1.1e-23 | 0009s15430 | Carotiniod oxygenase |
| # 211 | 607:7,883 | 160.0 | 3.2e-37 | 0607s00230 | No functional annotations for this locus | ||
| # 213 | 7:1,458,117 | 42.8 | 3.52e-3 | 0007s02460 | Ubiquitin-like protein | ||
| N82-11 | 1 | # 224 | 15:11,529,160 | 1103.1 | 0 | ||
| N82-14 | 2 | # 6 | 5:23,009,323 | 482.8 | 7.9e-135 | ||
| # 76 | 16:13,632,466 | 452.1 | 9.8e-126 | ||||
| # 4 | 2:10,051,122 | 42.8 | 1.6e-2 | ||||
| # 73 | 12:3,471,598 | 488.2 | 2.1e-136 | 0012s04240 | ABC-2 type transporter | ||
| # 28 | 19:12,716,418 | 223.1 | 1.3e-56 | 0019s11440 | Protein of unknown function (DUF803) | ||
| # 110 | 4:21,317,871 | 1402.5 | 0 | 0004s22400 | UDP-glucuronosyl and UDP-glucosyl transferase | ||
| N82-15 | 8 | # 131 | 7:13,340,488 | 389.0 | 8.8e-107 | 0007s13230 | Dolichyl pyrophosphate phosphatase and related acid phosphatases |
| # 137 | 2:10,620,751 and | 729.8 | 0 | ||||
| 2:10,621,198 | 268.2 | 1.8e-70 | 0002s14350 | PHD-finger | |||
| # 138 | 20:865,030 | 91.5 | 2.4e-17 | ||||
| # 140 | 2:21,231,185 | 524.3 | 2.1e-147 | 0002s23690 | Cysteine protease inhibitor activity | ||
| # 29 | 4:1,137,737 | 771.3 | 0 | 0004s01860 | Cytochrome P450 CYP2 subfamily | ||
| # 85 | 10:16,307,759 | 787.6 | 0 | 0010s17860 | Phosphoglycerate kinase [EC:2.7.2.3 ] | ||
| N92-1 | 6 | # 151 | 17:141,236 | 194.2 | 2.8e-48 | 0017s00430 | Serine/threonine protein kinase |
| # 155 | 8:6,405,007 | 403.4 | 5.0e-111 | ||||
| # 242 | 10:13,783,139 | 987.7 | 0 | 0010s14080 | Diaphanous homolog 2 | ||
| # 244 | 4:17,605,886 | 735.3 | 0 | ||||
| N92-4 | 1 | # 93 | 10:13,739,252 | 336.7 | 6.,3e-91 | ||
| N95-3 | 1 | # 179 | 9:9,182,685 | 253.8 | 4.2e-66 | 0009s10820 | Apoptosis-promoting RNA-binding protein TIA-1/TIAR |
| N95-6 | 2 | # 176 | 1:42,827,910 | 740.7 | 0 | ||
| # 190 | 1:2,422,398 | 459.3 | 8.0e-128 | 0001s03080 | Type II intron maturase | ||
Genomic insertion locus (scaffold and position) with score, e-value and, if applicable, annotated transcript.
In BLAST-analyses with more than one hit the one with lower e-value is shown.