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. 2011 Dec 20;11:126. doi: 10.1186/1472-6750-11-126

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Copyright ©2011 Andersen et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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In vitro neutralisation of anthrax LT by purified Lactobacillus produced scFv. (A) In vitro neutralisation by L. paracasei produced scFvs of anthrax LT in a macrophage toxicity assay (cell viability) visualised by staining with MTT. Comparison of neutralising capabilities of the secreted 1H scFv produced by KKA308 and the attached 1H scFv produced by KKA317 with the corresponding secreted and attached SAI/II as negative controls. (B) Quantification of in vitro neutralisation by colorimetric measurement of the break down of MTT into dark blue MTT-formazan.