Table 3.
Induction of Interferon-Stimulated Genes downstream of TEL-PDGFRB.
| Gene Description | Fold-induction |
|---|---|
| Chemokine (C-X-C motif) ligand 10 (Cxcl10) | 234.5 |
| Guanylate nucleotide binding protein 1 (Gbp1) | 103.1 |
| Guanylate nucleotide binding protein 2 (Gbp2) | 53.0 |
| Signal transducer and activator of transcription 1 (Stat1) | 19.0 |
| Interferon-regulatory factor 7 (Irf7) | 14.6 |
| Myxovirus-resistance 1 (Mx1) | 10.1 |
| Isg15 ubiquitin-like modifier (Isg15) | 8.2 |
| HLA, Class I, G (Hla-g) | 7.2 |
| Proteasome subunit, beta type 9 (Psmb9) | 6.3 |
| Proteasome subunit, beta type 10 (Psmb10) | 5.7 |
| Lectin, galactoside-binding, soluble, 3 binding protein (Lgals3bp) | 4.6 |
| Caspase 4 (Casp4) | 4.4 |
| Interferon-induced protein with tetratricopeptide repeats 2 (Ifit2) | 4.3 |
| Nicotinamide phosphoribosyltransferase (Nampt) | 4.3 |
| Interferon-regulatory factor 1 (Irf1) | 4.2 |
| Tyrosyl tRNA synthetase (Yars) | 4.1 |
| Tripartite motif containing 21 | 3.7 |
| N-myc and Stat interactor (Nmi) | 3.3 |
| Pml transcription factor (Pml) | 3.1 |
| Tryptophanyl tRNA synthetase (Wars) | 2.8 |
| Eif2 alpha kinase 3 (Eif2ak3) | 2.6 |
| Proteasome subunit, beta type 8 (Psmb8) | 2.4 |
| Proteasome 28 subunit, alpha (Psme1) | 2.3 |
| Interferon regulatory factor 9 (Irf9) | 2.2 |
| Protein phosphatase 5, catalytic subunit (Ppp5c) | 2.2 |
Genes regulated by TEL-PDGFRB that were previously reported to be ISGs were identified. Data represent the fold-changes in gene expression at steady state obtained from the microarray experiment
All three genes were induced by TEL-PDGFRB as confirmed by Q-PCR (Table 2). Interestingly, the expression of Cxcl10 was similarly elevated downstream of BCR-ABL, whereas the induction of Stat1 and Gbp1 was observed uniquely downstream of TEL-PDGFRB (see summary in Table five).