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. 2012 Mar 6;7(3):e32603. doi: 10.1371/journal.pone.0032603

Figure 5. Identification of the complexin-SNAREpin complex in the non-raft fractions.

Figure 5

(A) A Q-SNARE complex containing syntaxin 3 and SNAP 23 was detected on Western blots of the high sucrose gradient fractions (#11–13, indicated with number signs). This complex was found to lack the complementary R-SNARE VAMP 2 and thus enabled us to distinguish two different SNARE containing complexes in the porcine sperm. (B) The previously identified SNARE complex responsible for acrosome to plasma membrane docking (containing syntaxin 1B/SNAP 23/VAMP 3 [8]) was not observed in the same fractionated DRM sample at the high M.W. position (at 75 kDa) indicating the dissociation of this SNARE complex upon AE. Arrowheads indicate monomeric form of SNARE proteins present in these fractions. The arrows indicate the expected molecular weight of protein on the Western blot.