Table 1. LC-MALDI MS-based identification of proteins from MVs derived from acrosome reacted porcine sperm.

Protein Name	Peptide Count	Accession Number	Sequence 1	Expect Value 1	Sequence 2	Expect Value 2	Database
Epididymal sperm-binding protein 1	9	ESPB1_PIG	AVYDGQWK	3,4E-05	YCLIEDYPR	5,0E-05	Swiss Prot v.57.10
Zona pellucida-binding protein 1*	5	ZPBP1_PIG	FFNQQVEVLGR	5,4E-07	IVGSPNFPVK	6,5E-05	Swiss Prot v.57.10
Carbohydrate-binding protein AWN	5	AWN_PIG	IFNSDGPQK	8,1E-06	QTIIATEK	3,4E-05	Swiss Prot v.57.10
Carbohydrate-binding protein AQN-3	4	AQN3_PIG	GSDDCGGFLK	2,1E-07	NYSGWISYYK	2,6E-07	Swiss Prot v.57.10
Acrosomal protein SP-10	2	ASPX_HUMAN	NQSFCNKI	2,6E-03	KIFEGGK	4,3E-03	Swiss Prot v.57.10
Sperm adhesion molecule 1 (SPAM 1)	2	Q8MI02_PIG	ESTALFPSIYLN	2,2E-04	QSIELVQQK	2,4E-02	Trembl v.40.10
Sperm inner acrosomal membrane protein IAM38*	2	Q2PMM0_BOVIN	FFNQQVEVLGR	1,2E-06	VYVMLHQK	5,1E-03	Swiss Prot v.57.10

Presented is a subset of the proteins identified for capacitated and acrosome reacted cells, that corresponds to sperm proteins previously reported in the literature to be relevant for sperm-oocyte interactions. Asterisks indicate identified acrosomal membrane proteins, others are plasma membrane specific.

^*indicates acrosomal specific membrane proteins.