Table 3. LC-MALDI MS-based identification of proteins from membrane cavitates from capacitated porcine sperm.
| Protein Name | Peptide Count | Accession Number | Sequence 1 | Expect Value 1 | Sequence 2 | Expect Value 2 | Database |
| Zonadhesin# | 17 | ZAN_PIG | FVELQTAFGLR | 2,8E-07 | QEGVSCLSK | 8,1E-06 | Swiss Prot v.57.10 |
| Epididymal sperm-binding protein 1# * | 9 | ESPB1_PIG | NCIVEGSFFGK | 3,2E-06 | TNSLSPWCATR | 1,6E-06 | Swiss Prot v.57.10 |
| Carbohydrate-binding protein AWN# * | 6 | AWN_PIG | SSSNIATIK | 5,7E-07 | QTIIATEK | 3,0E-06 | Swiss Prot v.57.10 |
| Carbohydrate-binding protein AQN-3# * | 5 | AQN3_PIG | GSDDCGGFLK | 3,9E-07 | NYSGWISYYK | 2,6E-06 | Swiss Prot v.57.10 |
| ADAM3b# | 4 | A5HJZ3_PIG | GLLCVSAQLR | 1,8E-04 | NFDTQYTYYK | 1,3E-06 | Trembl v.40.10 |
| Fertilin beta# | 4 | Q866A8_PIG | CHPNDLR | 9,4E-03 | TDESGACGLTA | 1,2E-09 | Trembl v.40.10 |
| PDZ domain-containing protein C16orf65 | 4 | CP065_BOVIN | DINCDVMIHR | 2,4E-06 | APSPYWTMVK | 4,0E-03 | Swiss Prot v.57.10 |
| Acrosomal protein SP-10# * | 3 | ASPX_HUMAN | NQSFCNKI | 5,2E-04 | KIFEGGK | 2,6E-02 | Swiss Prot v.57.10 |
| ADAM3a# | 3 | A5A4F6_PIG | QCAELFGK | 1,1E-03 | SEVVPFK | 1,6E-02 | Trembl v.40.10 |
| CD44 | 3 | CD44_BOVIN | YAGVFHVEK | 1,2E-04 | TEAADLCK | 1,5E-04 | Swiss Prot v.57.10 |
| Carbohydrate-binding protein AQN-1# | 2 | AQN1_PIG | ISTYEGPK | 1,3E-04 | EYVEVQDGLP | 8,0E-03 | Swiss Prot v.57.10 |
| Ras-related protein Rab-2A | 2 | RAB2A_HUMAN | GAAGALLVYDI | 1,5E-04 | MITIDGK | 2,2E-02 | Swiss Prot v.57.10 |
| Sperm adhesion molecule 1 (SPAM1)# * | 2 | Q8MI02_PIG | ESTALFPSIYLN | 8,5E-04 | TFMQETLK | 6,6E-03 | Trembl v.40.10 |
| Syntaxin-binding protein 2 | 2 | Q2NL10_BOVIN | DLSHILK | 2,7E-03 | ADTPSLGEGP | 1,6E-03 | Swiss Prot v.57.10 |
| VAMP 3 | 2 | VAMP3_HUMAN | ADALQAGASQF | 4,3E-17 | LQQTQNQVD | 9,7E-03 | Swiss Prot v.57.10 |
| Syntaxin 12 | 1 | STX12_HUMAN | ISQATAQIK | 3,9E-02 | - | - | Swiss Prot v.57.10 |
Presented are subsets of those proteins identified that correspond to previously identified sperm proteins relevant to sperm-oocyte interactions [29]. SNARE-related protein identifications are highlighted in bold. In addition one proteins with a putative function in the recruitment of SNARE proteins into membrane rafts [26] is shown in red. Number signs indicate proteins that have been previously found in membrane cavitates of porcine sperm [29]. Asterisks indicate proteins that were identified in the MV preparations (Tables 1 and 2). Protein ID was considered to be conclusive when two or more peptides were identified (e<0.05) (all proteins above the line).
Protein ID was considered conclusive when two or more peptides were identified (e<0.05, all proteins above the line).
Highlighted in bold: SNARE proteins or SNARE interacting proteins.
Highlighted in red: Protein with putative function in the recruitment of SNARE proteins into the membrane raft area.
: indicate proteins which are routinely found in the apical plasma membrane cavitates of porcine sperm.
: indicate proteins that are recovered as plasma membrane proteins in the mixed vesicles.