Figure 3. Comparison of fluorescein and SRB signal-dissipation kinetics.

A) Onset times, and B) dissipation times (plots i to vii) of fluorescein (green) and SRB (red) signals at pH 4.5, in the absence of rimantadine (plots i to iii) or in its presence (plots iv to vi). Plots i and iii, from experiments carried out in the absence of rimantidine, show fluorescein dissipation kinetics for the “rimantidine-sensitive” and “rimantidine-insensitive” subpopulations, respectively; these subpopulations were identified and sorted as described in the caption to Fig. 2. The kinetics of the latter subpopulation (plot iii, Table I) were used to estimate the contribution of this component to the overall kinetics in the presence of rimantidine (plot iv, Table II, parameter f1). Subtraction of this contribution yielded the kinetics of rimantidine-sensitive events in the presence of the drug (plot vi, Table III). See Methods for details. Onset times in A were fit with either a single gamma distribution (plots i, ii, iii, v and vi and tables I and III) or a dual gamma distribution (plot iv and table II) (see Methods). Plot vii shows mean times calculated from data shown in plots i, ii, vi and v, respectively. Error bars represent the standard error of the mean, or, in the case of fluorescein-signal dissipation in the presence of rimantadine, errors estimated from the error of the fit-derived f1 parameter (see Methods). Plot viii in B shows the percent of the overall virion population represented respectively by plots i, ii, vi, and v.