Table 1.

Primer Sequences and Condition for Quantitative Real-Time Reverse Transcription–Polymerase Chain Reaction

Gene	Primer nucleotide sequence	Product size (bp)	Annealing temperature (°C)	Accession number
β-actin	5′-ATC GTG GGC CGC CCT AGG CA-3′ (forward)	243	52	NM_031144
	5′-TGG CCT TAG GGT TCA GAG GGG-3′ (reverse)
C/EBPα	5′-AAGGCCAAGAAGTCGGTGGA-3′ (forward)	189	55	NM_012524.2
	5′-CAGTTCGCGGCTCAGCTGTT-3′ (reverse)
PPARγ2	5′-CGGCGATCTTGACAGGAAAG-3′ (forward)	174	59	AB019561
	5′-GCTTCCACGGATCGAAACTG-3′ (reverse)
Col2A1	5′-ATGACAATCTGGCTCCCAACACTGC-3' (forward)	364	55	BT007205
	5′-GACCGGCCCTATGTCCACACCGAAT-3′ (reverse)
Acan	5′-CTTGGGCAGAAGAAAGATCG-3′ (forward)	158	58	J03485
	5′-GTGCTTGTAGGTGTTGGGGT-3′ (reverse)
Tnmd	5′-GTGGTCCCACAAGTGAAGGT-3′ (forward)	60	52	NM_022290.1
	5′-GTCTTCCTCGCTTGCTTGTC-3′ (reverse)
Scx	5′-AACACGGCCTTCACTGCGCTG-3′ (forward)	102	58	NM_001130508.1
	5′-CAGTAGCACGTTGCCCAGGTG-3′ (reverse)
Alpl	5′-TCCGTGGGTCGGATTCCT-3′ (forward)	85	58	NM_013059.1
	5′-GCCGGCCCAAGAGAGAA-3′ (reverse)
Bglap	5′-GAGCTGCCCTGCACTGGGTG-3′ (forward)	263	60	M23637
	5′-TGGCCCCAGACCTCTTCCCG-3′ (reverse)