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. 2011 May 24;21(5):710–720. doi: 10.1089/scd.2011.0085

FIG. 4.

FIG. 4.

Distribution of H3K27me3 within interphase nuclei and GOWT1 mESC colonies. (A) H3K27me3 was detected at the nuclear periphery and the periphery of mESC colonies cultivated 24 h after passaging (arrows). Quantification was done using Leica TSC SP-5X software, according to the selected line. Fluorescence intensity of Oct4 (green line in graph) and H3K27me3 (red line in graph; arrows in the graph indicate increased H3K27me3 at periphery of mESC colony). (B) Increased levels of H3K27me3 at the periphery of mESC colonies was also observed in cells cultivated 72 h after passaging. Lower right panel shows 3-dimensional projection of mESC colony. Bars represent 15 μm. H3K27me3, H3K27-trimethylation.