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. 2011 May 24;21(5):710–720. doi: 10.1089/scd.2011.0085

FIG. 6.

FIG. 6.

Nuclear pattern of Oct4 and selected histone-related proteins in GOWT1 and wt mESC cells. (A) Comparison of GFP-Oct4 pattern (green) with pattern of endogenous Oct4 protein (red) detected using a specific antibody. (B) Nuclear patterns of (a) endogenous Oct4 in R1 mESCs and (b) Nanog distribution within the cell nuclei of R1 mESC colonies. (C) Western blots showing Oct4, Nanog, cyclin A, Cdk4, and lamin B levels in selected G1 and G2 cell cycle phases of R1 and D3 wild-type mESCs. Cylin A level was determined to confirm efficiency of cell sorting. Oct4 level was quantified by densitometry and results are shown in bar chart. (D) Oct4 and Nanog levels compared in GOWT1 and R1 cell lines according to intensity of fluorescence, quantified by LEICA LAS AF software (version 2.1.2).