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. Author manuscript; available in PMC: 2013 Jan 26.
Published in final edited form as: Oncogene. 2011 Nov 21;31(30):3547–3560. doi: 10.1038/onc.2011.514

Figure 1.

Figure 1

PEA-15 enhances H-Ras mediated epithelial cell transformation in vitro in an adhesion-independent manner. (A) The number of colonies formed by stably transfected iBMK cells in soft agar colony formation assays was analyzed after 14 days of incubation. (B) The colony diameter of colonies formed by stably transfected iBMK cells in soft agar after 14 days of incubation was measured. (C) Brightfield images shown depict average size and morphology of colonies formed by stably transfected cells (H-Ras and HR-PEA) in soft agar colony formation assays. White scale bars represent 200μm. (D) Immunoblot analysis of iBMK cell lines stably transfected with H-Ras and/or PEA-15. Membranes were probed for H-Ras and PEA-15 expression, respectively.