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. Author manuscript; available in PMC: 2013 Jan 26.
Published in final edited form as: Oncogene. 2011 Nov 21;31(30):3547–3560. doi: 10.1038/onc.2011.514

Figure 3.

Figure 3

PEA-15 alters expression of cell cycle regulatory proteins and increases activation and expression levels of MAPK pathway members. (A) Western blot analysis of cell cycle regulatory proteins (left panel) or MAPK pathway members (right panel) in stably transfected iBMK cell lines kept in suspension for 48h. (B) Western blot analysis of cell cycle regulatory proteins and MAPK pathway members in transiently transfected iBMK cells kept in suspension for 18h. (C) mRNA expression analysis of cyclin D and cdk4 in transiently transfected iBMK cells kept in suspension for 18h. Shown are the relative mRNA expression levels compared to the housekeeping gene. Statistically significant differences are marked with asterisks (* p<0.05, ** p<0.01, *** p<0.001). (D) Immunoblot of CHOK1 cells transiently transfected with ponasterone inducible PEA-15 (pIND-PEA15), vector control (pIND), PEA-15 or Raf. Cells were serum-starved and then treated with ponasterone or vehicle control and immunoblotted for expression of phosphorylated ERK, total ERK and PEA-15.