Figure 2.

Quantification of synaptophysin signal. Plot of p‐nitrophenyl phosphate (PNPP₁₀₀) absorbance values and best fit line from linear regression. PNPP₁₀₀ absorbance values for one‐fourth, one‐half, one, two, or four sections of a given tissue block that were adhered either on glass slides (●, Case 5 in Table 1) or nitrocellulose membranes (■, Case 18 in Table 1) and processed simultaneously. Linear regression showed a near perfect linear relationship (R² = 1.00) for both glass slides and membranes over this 16‐fold range. Inset: Incubation time in PNPP solution for one‐fourth, one‐half, one, two, or four tissue sections to reach absorbance = 1.00 also was linear (R² = 1.00) for tissue on both slides and membranes.