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. 2012 Mar 7;102(5):1022–1031. doi: 10.1016/j.bpj.2012.02.008

Figure 2.

Figure 2

Immunolabeling of mutant proteins in HeLa cells. All three mutants shown here (Cx50D3E, Cx50G8E, and Cx50GNI) were able to reach the cell surface and cell-cell interfaces (arrows). Intracellular fluorescent signals were also detected. The cellular localization of these mutants was indistinguishable from that of the wild-type Cx50. Nuclei were labeled with DAPI (blue). Phase-contrast images (right) show cell morphology. Scale bar, 20 μm.