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. 2012 Mar 7;102(5):1174–1183. doi: 10.1016/j.bpj.2012.01.040

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© 2012 by the Biophysical Society.

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Experimental K_d,app plots generated by SW-FCCS. The linear fits (dashed lines) serve as a guide to the eye. (a) Measurements of mRFP-Cdc42 + EGFP-IQGAP1 (solid circles) and; measurements of mCherry-Cdc42 + EGFP-IQGAP1 (crosses). Their average K_d,app are 382 ± 136 nM and 233 ± 92 nM, respectively. (b) Twice the amount of unlabeled Cdc42 compared to labeled Cdc42 was transfected together with mCherry-Cdc42 + EGFP-IQGAP1 (solid circles). This was compared with the measurement done in cells with only mCherry-Cdc42 + EGFP-IQGAP1 (crosses). The average K_d,app determined in the presence of the unlabeled competitor is 446 ± 152 nM.