Figure 1.

Superoxide flashes in cardiomyocytes. (A) Time-lapse confocal-frame (xyt) images of an adult rat cardiomyocyte expressing the superoxide probe mt-cpYFP. Arrows indicate individual mitochondria undergoing a flash. N, nucleus. (B) Space-time (xt) plot of a typical flash in A. Pseudolinescan image was reconstructed from the xyt data set, with the line placed across the long axis of the mitochondrion. (C) Normalized image (F/F₀) from B along with the spatial (right trace) and temporal (bottom trace) profiles of the flash.