Figure 2.

AFM images of AS aggregation intermediates. (A and B) Fuzzy balls. (C–E) Latent acunas. (F–N) Productive acunas. (O–Q) Fuzzy fibers. (R) Denuded fibers. (S) Mature amyloid fibrils. xy/z scale bars (nm): 200/10 (A), 100/7 (B), 250/20 (C), 100/25 (D), 200/35 (E), 100/30 (F), 1000/40 (G), 400/40 (H), 200/40 (I), 1000/40 (J), 500/40 (K), 200/20 (L), 300/30 (M), 100/20 (N), 200/20 (O), 200/20 (P), 100/20 (Q), 100/50 (R), and 100/50 (S). Coding: wtAS (magenta solid circles), wtAS + 2.5%AS-MFC (orange solid circles), 4°C (blue solid circles), 37°C (green solid circles), 70°C (red solid circles); in all cases 150 μM protein, 25 mM Na-PO₄ pH 6.2, 0.02% NaN₃. All samples (except E) were spin-coated (see Materials and Methods for details).