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Representative single-molecule FRET trajectories (left) and histograms (right) from selected mutants HP1-C7A::HP2 (A), HP1-C1A::HP2 (B), and HP2-A4C::HP1-U4G (C), which allowed the formation of a GC basepair by replacing the AU basepair. Vertical arrows indicate the photobleaching point for each trace.
