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Effect of pPDM-cross-linking on EPR spectra of spin-labeled myosin LCD in skinned fiber bundles. Spectra were acquired in relaxation (magenta), rigor (black), and the pPDM-cross-linked state (green). (Top) Conventional EPR of fibers oriented parallel to the field (V1, from Fig. 2). (Bottom) STEPR spectra (V2′) of fibers oriented perpendicular to the field. (Inset) ∫V2′. The buffer was RS190 (rigor and pPDM) or relaxation solution (see Methods).
