Figure 6.

Cycs enhancer-driven GFP expression in hippocampal neurons requires intact CREB and NRF1 binding sites.(A) Box and whisker plots showing the relative levels of steady state GFP expression in neurons infected with reporter viruses containing mutations in the CREB or NRF1 binding sites or both. Images of a least 25 cells in 10 fields were analyzed. Mean pixel intensities are in arbitrary units. Between group statistical significance was determined using the paired sample Wilcoxon signed rank test, ^***p < 0.001. (B) Western blot showing effect of bicuculline and TTX on mutated enhancers. NRF1⁻ enhancer (48 h TTX), NRF1⁻ enhancer (48 h bicuculline), CRE⁻ enhancer (48 h TTX), CRE⁻ enhancer (48 h bicuculline), intact enhancer (48 h TTX); intact enhancer (48 h bicuculline). Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as a loading control.