Figure 8.

Increase in Cycs CRE⁻ enhancer-driven GFP expression by bicuculline is dependent on ERK1/2 activation.(A) Box and whisker plots showing the relative levels of GFP expression in neurons infected with the CRE⁻ reporter virus after adding TTX, bicuculline, or bicuculline plus MEK1/2 inhibitor SL327 for 48 h. Images of a least 50 cells in 20 fields were analyzed. Mean pixel intensities are in arbitrary units. Between group statistical significance was determined using the paired sample Wilcoxon signed rank test, ^**p < 0.006. (B) Representative western blot of neuronal cultures treated with bicuculline for 8 and 48 h in the presence or absence of the MEK1/2 inhibitor SL327. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as a loading control.