Fig. 4.

Spectrum of filter cube camera attachment. Transmittance spectra of the excitation filter (magenta excitation), a 45° dichroic beam-splitting filter (45° blue reflective), and the barrier filters consisting of both long-pass yellow dichroic glass and short-pass cyan glass are shown. The dotted lines are extrapolations if only single barrier filters were used. The portion of the spectrum that excites red-shifted GFP (GFP excitation) is labelled. The regions of the spectrum corresponding to red-shifted GFP fluorescence (GFP fluorescence) and in planta chlorophyll fluorescence (chlorophyll fluorescence) are labelled.