Fig. 7.

CMOS and CCD detector response to red-shifted GFP fluorescence. Enhanced GFP (A and B: 79 ng mm⁻²; C: 32.7 ng mm⁻²) in blackened microtitre plate wells (A, inset) were photographed with exposure times ranging from 0.1 to 25 s with the LED illuminated filter cube (A and B: 14 550 lx; C: 20 200 lx). Both cameras were set with an ISO of 800 and an aperture of 8.0. Counts observed from wells (inset 1) containing dilution buffer (1× PBS) were subtracted from EGFP light intensity measurements (inset 2). (A) Canon camera with CMOS detector configured with the filter cube containing a combination of dichroic yellow glass and cyan dichroic glass as the barrier filter. (B) Same camera as (A), with only a yellow dichroic barrier filter. (C) Nikon camera with a CCD detector configured with a filter cube as described in (B).