Fig. 9.

Digital camera response to in planta GFP expression. A mixed population of 13-day-old peat-grown wild-type (Wt.) seedlings and transgenic seedlings expressing the CaMV35S/GFP chimeric gene (35S/GFP) were photographed with a CMOS-containing Canon camera with the filter cube attachment containing a barrier filter made of yellow dichroic glass (A) or a barrier filter made of a combination of yellow and cyan dichroic glass (B). The photographs had exposure times from 0.05 to 30 s. Photographic conditions were an aperture of 8.0, an ISO of 1600 and an illumination intensity of 11 660 lx. Pixel intensity measurements were taken from expanded cotyledons.