FIG. 6.

ITSCs are able to differentiate into neuro-ectodermal lineage (A) ITSCs were cultivated in neuronal differentiation medium for 4 weeks to induce neuronal differentiation. (B) Neuronally differentiated ITSCs showed typical neuronal morphology and co-expressed neuronal markers β-III-tubulin and Neurofilament-H. Nuclei were stained with TOTO. Scale bar: 20μm. (C) RT-PCR showed the downregulation of nestin and upregulation of synaptophysin, and the channels NMDA1, AMPA1, and the GABA transporter. (D) The functionality of differentiated neurons was proven by a synaptic vesicle recycling assay resulting in strong labeling with FM1-43 dye in several neurons, while no labeling was detectable in undifferentiated ITSCs. Scale bar: 50μm.