FIG. 7.

ITSCs differentiate into several cell types with mesodermal origin (A) Spontaneous differentiation revealed that ITSCs could be differentiated into mesodermal lineage demonstrated by αSMA-positive differentiated cells. (B) ITSCs can differentiate into adipogenic cell as demonstrated by Oil Red O staining. Scale bar: 50μm. (C) ITSCs, cultured as high density pellets showed acidic sulfated mucosubstances (blue) visualized Alcian blue staining after chondrogenic differentiation. Scale bar: 100 μm. (D) ITSCs, differentiated into osteogenic lineage showed high ALP activity and Osteogenic mineralization visualized by Von Kossa and Alizarin red S stainings. Scale bar: 200μm