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. 2011 Nov 30;21(5):742–756. doi: 10.1089/scd.2011.0419

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Copyright 2012, Mary Ann Liebert, Inc.

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FIG. 9. — ITSCs can be efficiently enriched by magnetic cell sorting (MACS) based on the expression of p75^NTR. (A) Before enrichment 30.4% p75^NTR expressing cells were determined. After MACS only 3.3% cells in the negative fraction and the majority of 76.2% cells in the positive fraction showed the expression of p75^NTR. (B, C) p75^NTR+ sorted ITSCs formed larger neurospheres and proliferate faster than the negative fractions. (D) Reverse transcriptase PCR analysis showed the expression of the stemness marker nestin and the “pluripotency factors” in both fractions, whereas Oct4A and p75 are only present in p75^NTR+ cells. cDNA was normalized using the housekeeping gene β-Actin.