Table 4.

Nucleotides at 12 informative sites in COI-COII sequences of 13 strains of D. lini and its sibling species (D. ohnishii and D. ogumai). Diagnostic nucleotides for DNA barcoding are indicated with an asterisk. The positions of nucleotide sites are based on the whole length of the COI (1536 bp) and COII (684 bp) sequences of D. yakuba [39]. See Table 3 for further explanations.

Position

Species

N

COI (sites 1407–1536)

COII (sites 1–639)

Phylogeny

1407

1485

1503

69

72

231

232

234

399

435

486

570

D. lini

7

C

Y

T

T

C

C

T

R

Y

W

A

R

Non-monophyletic

D. ohnishii

4

C

C

T

T

C

C

Y

A

T

A

A

A

Non-monophyletic

D. ogumai

2

Y

T

C*

C*

T*

T*

T

A

T

A

G*

A

Monophyletic