Table 3 . Interaction of sao-1 with lag-1 and glp-1 embryonic mutants.
| Genotype of mother | Temperature | % viable progeny (n) | ||
|---|---|---|---|---|
| sao-1(+) | sao-1(ik1) | sao-1(ok3335) | ||
| lag-1(om13)a | 20° | 4.4 (480) | 30 (502)** | 4.2 (1116) |
| 25° | 4.0 (1289) | 20 (868)** | 0.66 (911)** | |
| glp-1(e2144)a | 15° | 100 (161) | 99 (282) | 99 (928) |
| 20° | 99 (881) | 97 (800)* | 41 (392)** | |
| 23° | 3.2 (693) | 1.3 (634) | 0 (430)** | |
| 25° | 0 (238) | 0 (468) | 0 (253) | |
| glp-1(e2142)a | 15° | 94 (599) | 89 (555)* | 38 (445)** |
| 20° | 14 (273) | 2.8 (324)** | 0 (306)** | |
| 25° | 0 (262) | 0 (246) | 0 (>500) | |
| glp-1(bn18)a | 20° | 93 (580) | 84 (365)** | 17 (544)** |
| 23° | 6.2 (499) | 0 (477)** | 0 (>500)** | |
| 25° | 0 (>500) | 0 (>500) | 0 (>500) | |
| glp-1(ar202)a | 20° | 80 (426) | 90 (479)** | 47 (403)** |
| 23° | 68 (989) | 70 (735) | 22 (446)** | |
| + | 15° | 100 (275) | 100 (761) | 99 (1044) |
| 20° | 100 (288) | 99 (718) | 98 (588) | |
| 23° | 100 (309) | 99 (873) | 93 (1341)** | |
| 25° | 99 (569) | 97 (646)* | 60 (882)** | |
Embryos were collected from at least 10 mothers that were homozygous for either sao-1(+), sao-1(ik1) or sao-1(ok3335) and also homozygous for the indicated glp-1 or lag-1 allele; mothers represented in the last four rows have no mutation other than the indicated sao-1 allele. Mothers were raised at 20 °, shifted to the designated temperature as mid L4s, and acclimated for at least 24 hr before embryos were collected and raised at the same temperature (see Materials and Methods for details). Viable progeny are those that reach at least the L4 stage. n, number of embryos scored. A two-tailed Fisher exact test was performed for each sao-1(ik1) or sao-1(ok3335) value relative to the equivalent sao-1(+) value in the same row; asterisks indicate a statistically significant difference between the sao-1 mutant and the corresponding sao-1(+) embryos with P values as follows: *P < 0.01, **P < 0.0001.
Temperature-sensitive alleles for which 25 ° is the restrictive temperature at which embryonic viability is severely compromised (Priess et al. 1987; Kodoyianni et al. 1992; Qiao et al. 1995; Pepper et al. 2003).