Figure 3 .

(A) The center of significant calling card clusters is very likely to be located within 250 bp of an SP1 binding site and an SP1 ChIP-seq peak and shows a large enrichment relative to insertions directed by the native PB transposase or random genomic TTAA sites. (B) The highest information content motif in the sequences flanking the center of the SP1-directed clusters is the canonical SP1 binding site. (C) The center of the calling card peaks shows a distribution centered on the SP1 binding site. (D) The top clusters identified by two biological replicates of SP1-directed calling cards show a 72% overlap.