Figure 5 .

Ectopically expressed swi2S suppresses mc⁻ (CY195) and Δabp1 (CY327) mutants’ switching defect. (A) The mat1 M/P ratio of cultures was determined with the method described in Figure 2B. Analysis of an empty pREP3 vector transformant was included for comparison, as a negative control (wt, SP976). (B) Iodine-staining colony phenotype. Moderate swi2S gene expression was achieved by 0.05 μM thiamine addition to the medium and thiamine was not added to achieve overexpression.