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Nesprin-3 recruits intermediate filaments to the nuclear envelope in COS7 cells. COS7 cells stained for vimentin reveal the typical cytoskeletal staining (A-A′′). In HA-Nesprin-3 transfected COS7 cells vimentin was recruited to the NE and co-localized with HA-Nesprin-3 (B-B′′). Confocal images are shown. Size bar, 10 μm.
