Figure 4.

Ultrastructural changes in the HUVECs after treatment with 40 μg/mL of EENL or vehicle for 24 hours observed under TEM. Vehicle-treated cells were used as control. The experiments were repeated in duplicates, and the representative images were shown. (a) Vehicle-treated cells showing morphological structure of HUVECs at 1650x. (b) Mitochondria indicated as M, and the endoplasmic reticulum as ER, in the vehicle treated cells at 6700x. (c)–(f) Depict cells treated with EENL. (c) Morphological change showing elongated cells at 1650x. (d) Abnormally small round mitochondria with fewer dilated cristae at 2100x. (e) Multivesicular bodies indicated as V, in the cells undergoing deterioration at 3000x. (f) Fragmented nuclei indicated as N, and the vacuolization in cells at 1100x.