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. 2012 Feb 7;2012:861794. doi: 10.1155/2012/861794

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Copyright © 2012 Jennifer Mumaw et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Viability of MSCs on day 0 and 4 after encapsulation with and without cryopreservation: MSCs were stained with calcein AM for live (green) and ethidium homodimer for dead (red). On the day of microencapsulation ovine A MSCs stained for (a) live, (b) dead, and (c) overlay. And on day 4 following microencapsulation (e) ovine A MSCs stained for live, (f) dead, and (g) overlay. (d) Graphical representation of counts of 90 images. Cryopreserved ovine A encapsulated MSCs on day of thaw (i) live, (j) dead, and (k) overlay. Day 4 postthaw ovine A MSCs stained for (l) live, (m) dead, and (n) overlay. (h) Graphical representation of counts of 90 images (averaging 87,000 cells per group). (*P < 0.0001).