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Images acquired during the surgery to generate the orthotopic model of distal esophageal adenocarcinoma. Distal esophagus (panel A, white arrow) was exposed following a midline incision in the upper abdomen. The 32-guage needle was inserted into the esophagus wall (panel B, white arrow) and after injection, induction of a small bulge (panel C, white arrow) was considered as a sign of successful injection.
