Table 4.
Conjugational transfer of multidrug resistance phenotypes in E. coli
| Donor | Transconjugant | cMIC (μg/ml) of antibiotics towards donors and transconjugants | aConjugation frequency | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Resistance pattern | Plasmid (kb) | bESBL activity | Resistance pattern | Plasmid (kb) | bESBL activity | Am | C | Cf | Cfx | G | Nx | Co | ||
| Am, Cf, Ce, Cfx, Co, Of, Nx, Ca, C |
32.5 | - | Am, Cf, Cfx, Co, Of, Nx | 32.5 | - | D | > 512 | > 256 | > 512 | 128 | 512 | 0.3 × 10-7 | ||
| T | > 512 | 64 | > 512 | 64 | 512 | |||||||||
| Am, Cf, Ce, Cfx, Co, Of, Nx, Ca, C, G, Ci |
32.5 | + | Am, Cf, Cfx, Co, Of, Nx | 32.5 | + | D | > 512 | > 256 | > 512 | > 256 | > 512 | 0.6 × 10-7 | ||
| T | > 512 | 64 | 512 | 64 | > 512 | |||||||||
| Am, Cf, Co, Nx, Na, Of, Ca, Ci | 32.5 | + | Am, Cf, Cfx, Co, Of, Nx | 32.5 | + | D | > 512 | > 256 | > 512 | > 256 | > 512 | 1.5 × 10-7 | ||
| T | > 512 | 128 | > 512 | 64 | 512 | |||||||||
| Am, Cf, Ce, Cfx, Co, Of, Nx, Ca, G, Ci |
32.5 | - | Am, Cf, Cfx, Co, Of, Nx | 32.5 | - | D | 512 | > 256 | > 512 | > 256 | > 512 | 0.6 × 10-7 | ||
| T | 512 | 64 | 512 | 64 | 512 | |||||||||
| Am, Cf, Ce, Cfx, Co, Of, Nx, Ca, C, G, Ci |
32.5, 4.7 | + | Am, Cf, Cfx, Co, Of, Nx, C, G |
32.5 | + | D | > 512 | 512 | > 256 | > 512 | > 64 | > 256 | > 512 | 1.2 × 10-7 |
| T | > 512 | 512 | 128 | 512 | > 64 | 128 | > 512 | |||||||
| Am, Cf, Ce, Cfx, Co, Of, Nx, Ca, G, Ci |
51 | + | Am, Cf, Cfx, Co, Of, Nx | 51 | - | D | 512 | > 128 | 512 | 128 | 512 | 0.5 × 10-7 | ||
| T | 256 | 32 | 256 | 32 | 512 | |||||||||
| Am, Cf, Ce, Cfx, Co, Of, Nx, Ak, Ca, G, Ci |
38 | + | Am, Cf, Cfx, Co, Of, Nx | 38 | + | D | > 512 | > 256 | > 512 | > 256 | > 512 | 0.8 × 10-7 | ||
| T | > 512 | 64 | > 512 | 64 | > 512 | |||||||||
| Am, Cf, Co, Nx, Ca, Ci | 38 | - | Am, Cf, Cfx, Co, Of, Nx | 38 | - | D | 512 | 128 | > 512 | 128 | 512 | 0.5 × 10-7 | ||
| T | 256 | 64 | 256 | 64 | 512 | |||||||||
| Am, Cf, Ce, Cfx, Co, Of, Nx, C, Ca, G, Ci |
32.5, 6.5 | + | Am, Cf, Cfx, Co, Of, Nx | 32.5, 6.5 | + | D | 512 | 128 | > 512 | 128 | > 512 | 0.3 × 10-7 | ||
| T | 512 | 64 | > 512 | 64 | > 512 | |||||||||
| Am, Cf, Ce, Cfx, Co, Of, Nx, Ca, Ci |
32.5 | + | Am, Cf, Cfx, Co, Of, Nx | 32.5 | + | D | > 512 | 128 | > 512 | 128 | > 512 | 1.2 × 10-7 | ||
| T | > 512 | 64 | > 512 | 64 | 512 | |||||||||
aConjugation frequency is the number of transconjugants per donor cell
bESBL = extended-spectrum β-lactamase
cMIC = minimum inhibitory concentration of antibiotic at which visible growth of isolates were inhibited
Am-amoxycillin, Ce-cefotaxime, Cfx-cefixime, Cf-ciprofloxacin, Co-cotrimoxazole, Nf-nitrofurantoin, Nx-norfloxacin, Of-ofloxacin, Ak-amikacin, C-chloramphenicol, Ca-ceftazidime, Ci-ceftriaxone, G-gentamycin, D-donor, T-transconjugant