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Effect of ATP7A-siRNA 80 h on ATP7A protein expression in A549/DDP ells. Equal loading was confirmed with GAPDH as a control. Western blot analysis of ATP7A in A549/DDP cells following treatment with control siRNA and different concentrations of targeted ATP7A siRNA (25 nM, 50 nM and 100 nM) for 80 h. Protein expression was quantified by Quantity One software. Results are the representative of three similar experiments.
