Table 2.
Oligonucleotides used for the mutations of Dlk2 GC-boxes
| PLASMID | OLIGONUCLEOTIDES | SEQUENCE |
|---|---|---|
| pGL3Basic(-212/+1M1) | Sp1212/1M1F | 5'- GTGCGCAGCGGGGGTGGATATAAGGCGCAGTCGGTGCGGG -3' |
| Sp1212/1M1R | 5'- CCCGCACCGACTGCGCCTTATATCCACCCCCGCTGCGCAC -3' | |
| pGL3Basic(-212/+1M2) | Sp1212/1M2F | 5'- GGGCGCAGTCGGTGCGGAATAAGCCGCGGCGGCGGGAGG -3' |
| Sp1212/1M2R | 5'- CCTCCCGCCGCCGCGGCTTATTCCGCACCGACTGCGCCC -3' | |
| pGL3Basic(-212/+1M3) | Sp1212/1M3F | 5'- GGCTCCGCCGGCACACGTTATTCCTGGCAGGGGGCCGAG -3' |
| Sp1212/1M3R | 5'- CTCGGCCCCCTGCCAGGAATAACGTGTGCCGGCGGAGCC -3' | |
| pGL3Basic(+1/+177M4) | Dlk2MluI-400U | 5'- CCCGCGGCCAGCTAGGTTATTCCCCCCTCCCGCCCCC -3' |
| Dlk2HindIII-1L | 5'- GGGGGCGGGAGGGGGGAATAACCTAGCTGGCCGCGGG -3' | |
| pGL3Basic(+1/+177M5) | Dlk2MluI-205U | 5'- AGGGGCGGCCCCCCTCAATAACCCGGCCCACGTCCGTC -3' |
| Dlk2HindIII-1L | 5'- GACGGACGTGGGCCGGGTTATTGAGGGGGGCCGCCCCT -3' | |
| pGL3Basic(+1/+177M6) | Dlk2 Xho-212U | 5'-GCCCCCCTCCCGCCCCAATTAACAGTCCGTCGGATCCG-3' |
| Dlk2HindIII-1L | 5'- CGGATCCGACGGACTGTTAATTGGGGCGGGAGGGGGGCC -3' | |