Table 3.
Oligonucleotides used for EMSA
| OLIGONUCLEOTIDE | SEQUENCE | |
|---|---|---|
| Sp1-A | Sp1-As | 5-GCTCCGCCGGCACACGCCGCCCCTGGCAGGGGGCCGAGCGC-3 |
| Sp1-Aas | 5-GCGCTCGGCCCCCTGCCAGGGGCGGCGTGTGCCGGCGGAGC-3 | |
| Sp1-B | Sp1-Bs | 5-TGCGCAGCGGGGGTGGGCGCGGGGCGCAGTCGGTGCGGGGCGGGCCGCGGCGGCGGGAGG-3 |
| Sp1-Bas | 5-CCTCCCGCCGCCGCGGCCCGCCCCGCACCGACTGCGCCCCGCGCCCACCCCCGCTGCGCA-3 | |
| Sp1-C | Sp1-Cs | 5-GGCCAGCTAGGGGCGGCCCCCCTCCCGCCCCCCGGCCCAG-3 |
| Sp1-Cas | 5-GTGGGCCGGGGGGCGGGAGGGGGGCCGCCCCTAGCTGGCC-3 | |
| M4 | M4s | 5-GGCCAGCTAGTTTATTCCCCCCTCCCGCCCCCCGGCCCAG-3 |
| M4as | 5-CTGGGCCGGGGGGCGGGAGGGGGGAATAAACTAGCTGGCC-3 | |
| M5 | M5s | 5-GGCCAGCTAGGGGCGGCCCCCCTAAATAACCCCGGCCCAG-3 |
| M5as | 5-CTGGGCCGGGGTTATTTAGGGGGGCCGCCCCTAGCTGGCC-3 | |
| M6 | M6s | 5-GGCCAGCTAGGGGCGGCCCCCCTCCCGCCCCAATTAAAAG-3 |
| M6as | 5-CTTTTAATTGGGGCGGGAGGGGGGCCGCCCCTAGCTGGCC-3 | |
| MT | MTs | 5-GGCCAGCTAGTTTATTCCCCCCTAAATAACCAATTAAAAG-3 |
| MTas | 5-CTTTTAATTGGTTATTTAGGGGGGAATAAACTAGCTGGCC-3 | |
| Sp1 | Sp1s | 5-ATTCGATCGGGGCGGGGCGAGC -3 |
| Sp1as | 3-TAAGCTAGCCCCGCCCCGCTCG-5 | |
| Sp1Mut | Sp1Muts | 5-ATTCGATCGGTTCGGGGCGAG C-3 |
| Sp1Mutas | 3-TAA GCTAGCCAAGCCCCGCTCG-5 | |
Sp1 consensus (Sp1) and Sp1 mutant consensus (Sp1Mut) oligonucleotides were purchased from Santa Cruz Biotechnologies.