Table 3.
Comparison of the reliability of estimating A-to-I RNA editing of the Dα6 subunit using the peak height ratio method between Dα6IR2 and Dα6285F primers at different rates of expected editing
| Editing Site | ||||
|---|---|---|---|---|
| Seq. Primer | 398 | 400 | 415 | 416 |
| Dα6IR2 | 0.98 ± 0.03 (1.00) |
1.00 ± 0.03 (1.00) |
0.97 ± 0.05 (1.00) |
0.97 ± 0.05 (1.00) |
| Dα6285F | 0.94 ± 0.10 (0.99) |
0.88 ± 0.10* (0.99) |
0.80 ± 0.07* (0.99) |
0.85 ± 0.04* (1.00) |
Values are the slopes and associated confidence intervals of editing estimate vs. expected values. Numbers in parentheses indicate the r2 value. Slopes with * indicate values that are significantly different than 1.0 (i.e., 95% CI does not include 1.0)