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. 2012 Mar 7;7(3):e33316. doi: 10.1371/journal.pone.0033316

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Bonvicini et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Analysis of Parvovirus B19 genome sequence for the presence and distribution of CpG dinucleotides was performed by means of EMBOSS CpGPlot. Submission consisted of a consensus sequence of B19 genome, genotype 1, using standard parameters (Window length 100, Obs/Exp CpG ratio 0.6, Min C+G 50%, Min Length 200). Results are shown for positive sense (A) and complementary (B) strands. Upper graphs, distribution of observed/expected ratios of CpG dinucleotides; middle graphs, distribution of C+G; lower graphs, identification of putative CpG islands according to set parameters. (EMBOSS CpGPlot accessed at http://www.ebi.ac.uk/Tools/emboss/cpgplot/).