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. 2012 Mar 7;7(3):e33316. doi: 10.1371/journal.pone.0033316

Figure 3. CpG methylation and EGFP reporter gene expression in UT7/EpoS1 cells.

Figure 3

Activity of viral P6 promoter, as determined by direct detection of EGFP fluorescence following transfection in UT7/EpoS1 cells of: unmethylated (A) or in vitro methylated (B) pEGFP-P6 plasmid DNA; linear products derived from ligation of the viral promoter (unmethylated, C and E, or methylated, D and F), upstream of the reporter gene in pEGFP-1 vector backbone (unmethylated, C and D, or methylated, E and F). Transfected UT7/EpoS1 cells were harvested at 72 hpt, about 50000 cells were spotted on glass slides, fixed with 4% paraformaldehyde in PBS, and counterstained with Evans Blue. FITC filter set, original magnification 40×.