Table 3. The competition analysis of the transmission efficiency for the transmitted/founder viruses and chronic HIV-1 isolates.
Viral pairs | Variant(co-receptor) | Inoculum viruses (TCID50) | Transmission efficiency* |
BAL and CH040 | BAL (R5) | 3.0×105 | 1.72% |
CH040(R5) | 1.0×106 | 1.68% | |
BAL and CH058 | BAL (R5) | 1.0×104 | 0.44% |
CH058(R5) | 0.6×104 | 0.63% |
HIV-1 CH040 and CH058 are transmitted/founder viruses and HIV-1 BAL is a chronic isolate.
*The transmission efficiency was calculated by dividing the viral RNA copies in the inoculum with the viral RNA copies of the transmitted variants from the bottom wells. Real-time RT PCR with specific primers/probes set which could discriminate CH040, CH058 and BAL viruses was employed.