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. 2012 Mar 7;7(3):e32718. doi: 10.1371/journal.pone.0032718

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Ong et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Myotubes were incubated with incremental concentrations of CGA for 24 hours. A: Viability of myotubes was measured using MTT staining. Readings are expressed as a percentage of non-viable cells compared to vehicle-treated myotubes. B: Numbers of cells in cell-cycle phases were examined using propidium iodide staining and FACS analysis. Readings are expressed as percentage of cells stained by propidium iodide at different phases. Results are the mean ± SE of three independent experiments. *P<0.05 compared with vehicle-treated control.