|
E. coli
strains:
|
|
|
| JE28 |
MG1655::rplL-his
|
[27]
|
| Tuner |
F−
ompT hsdS
B (rB
− mB
−) gal dcm lacY1
|
Novagen |
| Tuner(DE3) |
F−
ompT hsdS
B (rB
− mB
−) gal dcm lacY1(DE3)
|
Novagen |
|
Plasmids:
|
|
|
| pKTplaccI |
cI-lacZ fusion gene under lac promoter control |
[28]
|
| pProEX-HTb |
|
Invitrogen |
| pProEX-S1F |
pProEX encoding HIS- and FLAG-tagged protein S1 |
This study |
| pProEX-S1ΔD1F |
pProEX encoding HIS- and FLAG-tagged protein S187–557
|
This study |
| pProEX-S1ΔD2F |
pProEX encoding HIS- and FLAG-tagged protein S1Δ103–181
|
This study |
| pProEX-S1D1-2F |
pProEX encoding HIS- and FLAG-tagged protein S1194
|
This study |
| pProEX-S1D1F |
pProEX encoding HIS- and FLAG-tagged protein S1106
|
This study |
| pProEX-S1D2F |
pProEX encoding HIS- and FLAG-tagged protein S187–194
|
This study |
| pPro-S1F |
pProEX-S1F encoding FLAG-tagged protein S1 |
This study |
| pPro-S1ΔD1F |
pProEX-S1ΔD1F encoding FLAG-tagged protein S187–557
|
This study |
| pPro-S1ΔD2F |
pProEX-S1ΔD2F encoding FLAG-tagged protein S1Δ103–181
|
This study |
| pPro-S1D1-2F |
pProEX-S1D1-2F encoding FLAG-tagged protein S1194
|
This study |
| pPro-S1D1F |
pProEX-S1D1F encoding FLAG-tagged protein S1106
|
This study |
| pPro-S1D2F |
pProEX-S1D2F encoding FLAG-tagged protein S187–194
|
This study |
| pPro-S1ΔD2F |
pProEX-S1ΔD2F encoding FLAG-tagged protein S187–557
|
This study |
| pET22b |
|
Novagen |
| pET-ccS2 |
pET derivative encoding HIS-tagged protein S2α2
|
This study |
