Table 2. Synthetic oligonucleotides used in this study.

	Sequence*	Restriction sites	Binding region
B5fw	TATAGGCGCCGAATTC ATGACTGAATCTTTTGCTC	NarI, EcoRI,	rpsA from codon 1
D5fw	TATAGGCGCCGAATTC ATGAAAGCTAAACGTCAC	NarI, EcoRI,	rpsA from codon 87
G5rev	TATACTCGAGTTATTTTTCATCGTCATCCTTATAGTCAGCATCTTCGTAAGC	XhoI	rpsA until codon 106
H5rev	TATACTCGAGTTATTTTTCATCGTCATCCTTATAGTCCATGCCTTCCTGCAGGGTCCATGCCTTCCTGCAGG	XhoI	rpsA until codon 194
I5rev	TATACTCGAGTTATTTTTCATCGTCATCCTTATAGTCGCCTTTAGCTGCTTTG	XhoI	rpsA until codon 557
V14fw	[P]GCCGTTATCGAATCCGAAAAC		rpsA from codon 182
W14rev	[P]GTAAGCTTTTTCCAGCGTGATCC		rpsA until codon 102
J5	TATAGAATTCCTCGAG GGTCTGTTTCCTGTG	EcoRI, XhoI	pProEX-Htb specific primer used for site-directed mutagenesis
H4fw	TATACATATG AACCATCGCTGGCTGG	NdeI	rpsB from codon 93
I4rev	TATACTCGAG TTAGTCCGGCAGACCGC	XhoI	rpsB until codon 159

^*Restriction sites are highlighted in italics; sequences encoding the FLAG-tag are shown in bold, and sequences complementary to the template are underlined.