Table 1.
Quality Control Metrics from DNA Workbench
| Specimen | Age of specimen1 | Test Fluorochrome | Pass/fail | DLRSpread2 | Signal to Noise 3 Green | Signal to Noise 3 Red |
|---|---|---|---|---|---|---|
| bone marrow | 1 day | CY3 | Pass | 0.206803 | 65.848474 | 63.860383 |
| CY5 | Pass | 0.199919 | 62.305342 | 52.162212 | ||
| bone marrow | 9 days | CY3 | Pass | 0.14503 | 98.418512 | 76.505274 |
| CY5 | Pass | 0.142963 | 87.635554 | 88.5432 | ||
| bone marrow | 15 days | CY3 | Pass | 0.209813 | 44.387109 | 34.835231 |
| CY5 | Pass | 0.205444 | 53.479156 | 41.008914 | ||
| bone marrow | 26 days | CY3 | Pass | 0.283387 | 38.740122 | 43.751865 |
| CY5 | Pass | 0.257136 | 39.285321 | 42.250693 | ||
| bone marrow | 27 days | CY3 | Pass | 0.158672 | 85.404253 | 76.821745 |
| bone marrow | 36 days | CY3 | Pass | 0.181903 | 71.365826 | 75.92553 |
| CY5 | Pass | 0.192083 | 59.676813 | 56.174289 | ||
| cytogenetic specimen | 52 days | CY3 | Pass | 0.174283 | 123.04883 | 104.706454 |
| cytogenetic specimen | 1 year | CY3 | Pass | 0.333462 | 40.65296 | 49.663864 |
| CY5 | Pass | 0.193984 | 56.378169 | 43.05297 | ||
| cytogenetic specimen | 8 years | CY3 | Pass | 0.218933 | 47.366763 | 47.266465 |
| CY5 | Pass | 0.198984 | 57.315046 | 56.775467 |
DNA Workbench quality classifications are given below the table. Quality measurements classified as "excellent" are in bold print and the single "poor" measurement is underlined; all other measurements were classified "good". Pairs of duplicate experiments are grouped together.
1 length of time at 4°C for bone marrow or at -80°C for chromosome suspension in Carnoy's fixative.
2 Excellent: < 0.2; Good: 0.2-0.3; Poor: > 0.3
3 Excellent: > 100; Good: 30-100; Poor: < 30