Table 1.

Experimental variables for each experimental group

Experimental Groups	Food	Age	Social Experience	Mating Status	Sex
Male vs Female	Mo-Mo	10 days	Grouped	Virgins	Males or Females
Young vs Old	Mo-Su	8–10 days, 38 days (w1118ex), or 56 days (wCS10)	Grouped	Virgins	Females
Mated vs Virgin	Mo-Su	10 days	Grouped	Virgins or Mated	Females
Molasses vs Dextrose	Mo-Mo or Dx-Dx	10 days	Grouped	Virgins	Females
Switching to Sucrose	Mo-Su or Dx-Su	10 days	Grouped	Virgins	Females
Group vs Isolation	Mo-Mo	10 days	Grouped* or Isolated	Virgins	Females
Canton-S	Mo-Mo	10 days	Grouped	Virgins	Females
Inbred Raleigh Strains	Mo-Mo	10 days	Grouped	Virgins	Females

The different variables we used for each of the experimental groups are summarized in this table. Mo-Mo = molasses-based food for entire study including behavioral recording, Mo-Su = flies kept on molasses-based food from eclosion and then switched to sucrose food for behavioral recording, Dx-Dx = dextrose-based food for entire study including behavioral recording, and Dx-Su = flies kept on dextrose-based food from eclosion and then switched to sucrose food for behavioral recording. Age = the flies' age in days at the start of the behavioral recording. Social Experience is the condition the flies were kept in before sleep recording, grouped = 8 to 10 flies, grouped* = 30 flies and isolated = 1 fly. Mating status is the condition of the fly when recorded. Sex is the sex of the flies used in the experiment.