Figure 1.

Initial screening of PS-SPCLs for peptides stimulating intracellular Ca²⁺ increase in human neutrophils. Each panel shows the results obtained with the peptide pools with known amino acids at each of the six positions of the hexapeptide. The six positions were individually defined (O1, O2 etc.) by one of the 19 L-amino acids. The remaining five positions consist of mixtures (X) of the 19 L-amino acids (except for cysteine). Human neutrophils (1 × 10⁶ cells/assay) were used for each assay. Fluorescence ratio (340/38) was monitored as described under Methods. The results represent one of three independent experiments.