Figure 3.

Effect of simvastatin on SPC-induced RhoA activation. (A) hASCs were treated with 2 µM SPC for the indicated time periods. (C) hASCs were pretreated with serum-free medium containing 1 µM simvastatin or vehicles (0.1% DMSO) for 1 day and then treated with 2 µM SPC or vehicles for the indicated time periods. The amounts of RhoA in total lysates and membrane fractions were determined by Western blotting. (B, D) The densities of RhoA in membrane fractions were quantified and normalized to those of RhoA in total lysates. Data are expressed as mean ± SD (n = 4; ^*P < 0.05). (E) hASCs were pretreated with 1 µM simvastatin (0.1% DMSO) or vehicles for 1 day, followed by treatment with 2 µM SPC for 5 min. The amounts of RhoA in the whole cell lysates (Total RhoA) or the GTP-bound RhoA precipitated from the lysates were revealed by Western blotting. Representative data from three independent experiments are shown.